Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Therapeutic CMP-nonulosonates against multidrug-resistant Neisseria gonorrhoeae

doi: 10.4049/jimmunol.1901398

Figure Lengend Snippet: Incorporation of NulO into N. gonorrhoeae LOS. A. mAb 3F11 binding. mAb 3F11 binds to terminal lactosamine of LNnT; extensions beyond LNnT abrogates 3F11 binding (55). N. gonorrhoeae F62 ΔlgtD was grown in media alone or media containing 25 µg/ml of the indicated CMP-NulO. mAb 3F11 binding was detected by flow cytometry and median fluorescence was recorded. Binding is shown as percentage relative to 3F11 binding to unsialylated bacteria (three independent observations). Comparisons across groups, made by one-way ANOVA, showed significant differences were observed (F=43.76; P<0.0001). Pairwise comparisons, made by Tukey’s multiple comparisons test, are indicated. B. Incorporation of NulO as visualized by silver-stained SDS-PAGE gels. Protease K lysates of F62 ΔlgtD grown in media alone or media containing each of the indicated CMP-NulOs (100 µg/ml) were separated on 16% Tricine gels (Bio-Rad) and LOS was visualized by silver staining. Slower mobility relative to bacteria grown in media alone (No CMP-NulO) indicates addition of NulO.

Article Snippet: Cell lysates were separated on 16.5% Criterion Tris-Tricine gels (Bio-Rad) using Tris-Tricine Cathode buffer (Boston Bioproducts) and LOS was stained using the Bio-Rad Silver Stain kit.

Techniques: Binding Assay, Flow Cytometry, Fluorescence, Bacteria, Staining, SDS Page, Silver Staining

Journal: EMBO Reports

Article Title: Satellite cell-derived TRIM28 is pivotal for mechanical load- and injury-induced myogenesis

doi: 10.1038/s44319-024-00227-1

Figure Lengend Snippet: Reagents and tools table

Article Snippet: FITC Anti-Mouse IgG2b , Jackson Immunoresearch , Cat# 115-095-207 IHC: 1:200.

Techniques: Knock-In, Recombinant, shRNA, Plasmid Preparation, Blocking Assay, Sequencing, Electron Microscopy, Software, Microscopy, Laser-Scanning Microscopy, In Situ, Labeling

Journal: Scientific reports

Article Title: Evaluation of the antiviral activity of ultraviolet light and zinc oxide nanoparticles on textile products exposed to Avian coronavirus.

doi: 10.1038/s41598-023-36100-9

Figure Lengend Snippet: Figure 2. Characterization of ZnONPs. (a) Transmission electron microscopy shows the spherical morphology of ZnONPs with an average size of 30 nm. (b) UV vis spectrophotometry. (c) Elemental analysis by EDS.

Article Snippet: The electronic structure and optical properties of the nanoparticles were analyzed using UV vis spectrophotometry (Shimadzu, UV 1900, Tokyo, Japan), previously calibrated in the range from 370 to 800 nm.

Techniques: Transmission Assay, Electron Microscopy, Spectrophotometry